Mycobacterium-specific IFNγ secretion post-vaccination.

<p>Both the SO2 primary and the MVA.85A booster vaccine are immunogenic in rhesus macaques. Antigen-specific IFNγ secretion was measured after 3 days of in vitro stimulation of fresh peripheral blood lymphocytes along the vaccination phase. PPD-specific IFNγ secretion is depicted as group mean responses (+standard error) in time (A), and as individual responses with medians at the peak of the mean group response (B). Similarly, results upon stimulation with recombinant Ag85A are displayed as group means (+standard error) in time (C), and as individual responses (with medians) at peak (D), respectively. Colouring of group medians (panels A and C) is representing non-vaccinated controls in blue, BCG only in green, BCG/MVA.85A in magenta, and SO2 vaccination in red; group colouring is maintained throughout the paper for comparibility of data. Dot plots representing individual animals (panels B and D) are consistently coloured as follows: animals per treatment group were ranked from highest to lowest total gross pathology score (sum of lung, hilar LN and extra-thoracic scores) and then assigned the colours: blue, green, magenta, red, brown, black, respectively; individual colouring is maintained throughout for comparability. P-values are relative to non-vaccinated control treatment and represented under the x-axis by symbols as follows: ▪ for 0.1>p≥0.05, ★ for 0.05>p≥0.01, ★★ for 0.01>p≥0.001.