T cell responses to αDCIR.HIV5pep and αCD40.HIV5pep vaccines in MVA-primed Rhesus macaques.

PBMCs were harvested at the indicated time points and analyzed by IFNγ ELISPOT using pools of overlapping Gag Pol and Nef peptides corresponding to sequences carried by the DC-targeting vector (HIV5 peptides, shown in light grey), or pools of overlapping Gag, Pol and Nef peptides corresponding to sequences not carried by the DC-targeting vaccines but carried by MVA GagPolNef (non-HIV5 pep, shown in dark grey). (A) IFNγ ELISPOT data from animals primed with two administrations of MVA GagPolNef, then vaccinated with 250 μg αDCIR.HIV5pep (G1) or (B) αCD40.HIV5pep (G2) plus 1 mg poly-ICLC according to the schedule shown in Table 1 and as indicated by the gray and black arrows below the timeline shown in weeks. (C) and (D) IFNγ ELISPOT data for individual Gag, Pol and Nef peptide stimulations corresponding to sequences carried by the DC-targeting vectors (individual HIV5pep peptides are indicated by colors). Responses for each peptide are the average response per sample point. The X-axis shows sampling time in weeks. S1 Table and S2 Table show data corresponding to this figure. Supporting information S1 File contains the primary data.