Impaired number of CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells in CLP-induced Atg7^f/fCD4-Cre mice.

<p>Splenocytes obtained at 18h after surgery were stained for surface markers (CD4 and CD8) and analyzed by flow cytometry. Viable lymphocytes were gated by using forward scatter versus side scatter (excluding cell debris). The gated lymphocytes were further gated on the CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells. Representative histograms were shown percentage of lymphocytes (A). The absolute numbers of CD4⁺CD8⁻ and CD4⁻CD8⁺ T-cell subsets in the spleen were enumerated (B). Results obtained from 3 animals in each group are shown as mean ± SEM in the bar graph. Data are compared by one-way analysis of variance and Student-Newman Keul's test. CLP: cecal ligation and puncture. *<i>P</i><0.05 vs. respective sham mice. #<i>P</i><0.05 vs. CLP-induced Atg7^f/f mice.