Frequencies of 1B11⁺ CD4⁺ and CD8⁺ T cells increase during sepsis.

B6 mice were subjected to CLP and sacrificed at the indicated timepoints. Spleens were harvested and CD4+ and CD8+ T cells were assessed for staining with the S7 or 1B11 clones that recognize distinct glycoforms of CD43. A) Representative histograms of S7 and 1B11 staining on CD4+ and CD8+ splenic T cells. B) Summary data of S7 staining on CD4+ T cells harvested from spleens at indicated time points post-CLP or from sham mice revealed no significant changes over time. C) Summary data of 1B11 staining on CD4+ T cells revealed a significant increase from sham mice to septic mice at 120hrs (10.7±0.6 vs 22.2±0.7, p = 0.0009) as well as a significant increase between septic mice at 24hrs and 120hrs (10.2±0.7 vs 22.2±0.7, p = 0.002). D) Summary data of S7 staining on CD8+ T cells revealed a significant increase from sham to sepsis at 72hrs (91±0.9 vs 96.3±0.2, p = 0.02) that then decreased again at 120hrs (96.3±0.2 vs 91.2±1.3, p = 0.03). E) Summary data of 1B11 staining on CD8+ T cells revealed an increase from sham to sepsis at 72hrs (4.1±0.3 vs. 18.3±2.5 vs p = 0.004). Data shown are 4–6 mice per group, and are representative of 3 independent experiments. Statistical comparisons were performed by one-way ANOVA followed by Kruskall-Wallis non-parametric post-tests.