Decreased autophagy and increased apoptosis in CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells after CLP.

<p>Spenocytes were obtained at 4h, 9h, 18h and 24h after CLP. Gating strategy for CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells by flow cytometry analysis (A) Cells were initially gated on a forward- and side-sactter lymphocytes gate to exclude dead cells, monocytes and granulocytes. The gated lymphocytes were then selected for either CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells. Cyto-ID Green (B), acridine orange (C) and Annexin-V (D) staining were further analyzed by flow cytometry. Representative histograms were gated on CD4⁺CD8⁻ and CD4⁻CD8⁺ T cells. Values were shown as mean fluorescence intensities (MFI) for Cyto-ID Green/acridine orange and percentage for Annexin-V staining. In the histogram of Annexin-V staining, light gray histogram represents a staining control without adding Annexin-V. Results obtained from 5–6 animals in each group are shown as mean ± SEM in the bar graph. Data are compared by one-way analysis of variance and Student-Newman Keul's test. CLP: cecal ligation and puncture. *<i>P</i><0.05 vs. sham-operated mice.