Binding affinities of the ASOs for the on- and off-target sites of the SOD-1 minigene mRNA spiked into the denatured nuclear extract.

<p>Dissociation constants (K_d) were determined as described in in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101752#s2" target="_blank">Materials and Methods</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101752#pone.0101752.s003" target="_blank">figure S3</a>. Differences in the binding affinities (ΔK_d) between the two targets were calculated by dividing the K_d of the ASOs for the off-target sites by the K_d for the on-target sites.