Affinities of wild-type 933W repressor protein for synthetic 933W operators.

  • Tammy J. Bullwinkle (327418)
  • Daniel Samorodnitsky (327419)
  • Rayna C. Rosati (327420)
  • Gerald B. Koudelka (327421)
Publication date
February 2013

Abstract

<p>The affinities of 933W repressor for the substituted DNAs are expressed as the dissociation constants of these operators for 933W repressor. NS-DNA that does not contain a DNA sequence to which 933W repressor binds specifically. The affinity of 933W repressor for non-specific DNA is give as an intrinsic ‘per-site’ dissociation constant (K<sub>D</sub><sup>NS</sup> = K<sub>D</sub><sup>OBS</sup>[2L-n], where L is DNA length and n is site size <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034563#pone.0034563-JenJacobson1" target="_blank">[28]</a>). The protein concentrations used were corrected for activity.</p

Extracted data

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