CD4 T-cell autoreactivity to GRP78.

<p><i>A</i>.) Addition of GRP78 to PBMNC cultures increased CD4 T-cell proliferation (BrdU uptake) relative to media controls (no added protein) (n = 47), unlike addition of elastin split products (ESP). P_c denotes alpha level corrected for multiple comparisons. <i>B</i>.) GRP78-induced CD4 T-cell proliferation was greatest among cultures from smokers with emphysema (n = 34). Specific indices (SI) of proliferation were calculated as %CD4 T-cells incorporating BrdU in GRP78-supplemented cultures <i>minus</i> incorporation in concurrent media controls. <i>C</i>.) GRP78 also increased percentages of CD4 T-cells that produced IFN-gamma again, unlike effects of ESP. <i>D</i>.) IFN-gamma production was also greatest in the CD4 T-cells from the emphysematous smokers. Specific indices (SI) were calculated as %CD4 T-cells producing IFN-gamma production in GRP78-supplemented cultures <i>minus</i> that of concurrent controls.