The effects of anti-B2285 Abs on the function of CD4⁺ T cells isolated from MRL/lpr mice in vitro.

<p>(A) The levels of anti-dsDNA autoantibodies in the culture supernatant from the co-cultured system of CD4⁺ T and B cells. (B) The proliferative levels of collected CD4⁺ T cells in the co-cultured system. (C) The representative pictures of T-bet, GATA-3, RORγt and FOXP3 expression in CD4⁺ T cells. (D) The results of statistical analysis for the protein levels of T-bet, GATA-3, RORγt and FOXP3 in CD4⁺ T cells. (E) The mRNA levels of TNF-α, IFN-γ, IL-6, IL-10, IL-17, and TGF-β of CD4⁺ T cells in different groups. (F) Representative pictures from each treatment group for CD45RO expression. (G) The statistical analysis for the levels of CD45RO in different groups. *, p<0.05 vs Control (medium without CD3/CD28 Abs) group; **, p<0.05 vs CD3/CD28 Abs and CD3/CD28/KLH Abs group. Values are means ± SEM.