<i>NRF2</i> knockdown increases cellular accumulation of Pba.

<p>(A) The cells were incubated with Pba (2.5 µg/mL) for 6 h and then washed with PBS. Intracellular accumulation of Pba was monitored by confocal microscopy. BF, bright field; ×100 magnification (B) Intracellular accumulation of Pba (2.5 µg/mL, 6 h) was quantified using a Cell Insight system. The data are ratios with respect to the sc-MDA control and are reported as the mean ± standard error (SE) of 6 experiments. (C) Intracellular accumulation of Pba (0.25 and 0.5 µg/mL, 6 h) was assessed by fluorometric measurement in cell lysates. The data represent ratios with respect to each no PDT control are reported as the mean ±SD of 3 experiments. ^a<i>P</i><0.05 as compared with sc-MDA with PDT.