PBA resticts <i>Mtb</i> uptake and intracellular growth in PBMC and MDM.

<p>(A) Uptake of <i>Mtb</i> H37Rv by PBMC 4 hrs post-infection and intracellular growth 96 hr-post-infection in the presence of a 2-fold serial dilution of PBA. (B) <i>Mtb</i> uptake by MDM pre-treated with 4mM PBA for 48 hrs. (C) Phagocytic receptor expression (CD11b, CD11c, CD16 or CD35) on MDM following 48 hrs 4mM PBA treatment:percent of live cells expressing receptors and median fluorescent index (MFI), by flow cytometry. (D) Intracellular <i>Mtb</i> growth in MDM treated with a serial dilution of PBA 4 hrs post-infection, measured by CFU. (E) Seven day intracellular growth of 4mM PBA pre-treated <i>Mtb</i>, and control <i>Mtb</i>, grown in MDM ± 4mM PBA post-infection. Mean ± SD or min-max (box plots), n = 3–4 in triplicate for CFU and n = 8 for flow cytometry; 1-way ANOVA with Dunnett’s multiple comparisons test (A,E,F) or Paired t-test (B,C,D): *, P<0.05, ***, P<0.001.