BFRs cause transient increases in intracellular [Ca²⁺] levels in SH-SY5Y cells.

<p>(A) Shows the effects of TBBPA (20μM), DBPE (20μM) and HBCD (10μM) on the transient elevation of intracellular [Ca²⁺] levels in SH-SY5Y cells as monitored using Fluo3-AM. The arrow indicates the point of addition of the chemicals. The changes in relative fluorescence intensities (F/Fo) are indicative to changes in intracellular [Ca²⁺] concentrations and the traces are the averages of 10 individual cells with the field-of-view. The traces are typical of 3 replicate experiments. (B) Shows the dose-dependent effects of HBCD on the maximal (peak) elevation in intracellular [Ca²⁺] levels (presented as change in relative fluorescence). The data points are the mean±SD of 3 determinations at each concentration.