EGFR and PI3K inhibitors diminish PCN-enhanced NRF2 accumulation in the nuclei of A549 cells.

<p>A549 cells were exposed to the EGFR inhibitor AG1478 (<b>A</b>), the PI3K inhibitor LY294002 (<b>B</b>), or a combination of both AG1478 and LY294002 (<b>C</b>), or AKT or ERK inhibitors (<b>D</b>) for 90 min before the addition of PCN (12.5 µg/ml) for 12 hr. The expression of nuclear NRF2 was examined by monoclonal anti-NRF2 antibody and quantified by densitometry. The experiments were repeated three times with similar results. Densitometry data represent the mean ± SD from all three experiments. <i>*p</i><0.05 when cells that were exposed to PCN were compared to control cells exposed to the same volume of sterile water. **<i>p</i><0.05 when cells that were exposed to AG1478 or LY294002 or AG1478 plus LY294002, or AKT and ERK inhibitors were compared to cells exposed to PCN alone.