<i>A. tumefaciens</i> mediated transformation efficiency of <i>Pb18</i> yeast cells with cassettes under the transcriptional regulation of different promoters.

<p>(<b>A</b>). Three independent ATMT experiments were performed to verify if the transformation efficiency of <i>Pb18</i> yeast cells could be further improved by expressing the bifunctional selective marker under the transcriptional regulation of Prm_Act or Prm_GP43 in relation to Prm_CBP1. Data analysis showed non-significant differences (CBP1 vs. Actin, P = 0.17; CBP1 vs. GP43, P = 0.55). (<b>B</b>). The gMFI of the bifunctional selective marker “Shble::mCherry”, which was expressed under the transcriptional regulation of Prm_CBP1, Prm_Act or Prm_GP43, was determined for yeast cell populations in late log-phase growth. Control refers to yeast cell populations transformed with the cassette “Prm_CBP1::<i>Shble</i>::Ttr_GP43”. The gMFI values were normalized in respect to control yeast cell populations expressing only the ZeoR selection marker. (*, P = 0.01).