ATMT of <i>P. brasiliensis</i> to certify the Zeocin/ZeoR positive selection system and the terminal GP43 gene transcription region.

<p>(<b>A</b>). Four independent transformation experiments were performed by co-cultivating 1×10⁶ viable <i>Pb18</i> yeast cells with <i>A. tumefaciens</i> at different ratios. <i>A. tumefaciens</i> carried the “Prm_CBP1::<i>Shble</i>::Ttr_GP43” cassette to express the ZeoR selection marker (*, P = 0.0002; **, P = 0.001). (<b>B</b>). Three independent transformation experiments were performed by co-cultivating 1×10⁶ viable <i>Pb18</i> yeast cells with <i>A. tumefaciens</i> at a ratio of 1∶100 and recovering the yeast cells using two different selective media (*, P = 0.02).