<i>Sm</i>Hsf1 can drive transcription in a modified yeast one-hybrid system.

<p>Yeast cells expressing <i>Sm</i>Hsf1 fused to the Gal4 DNA binding domain (Gal4DBD-<i>Sm</i>Hsf1) were patched (A) or serially diluted (B and C, from 1 to 10⁻⁵) on different selective media to test the ability of <i>Sm</i>Hsf1 to activate transcription. The positive control yeast express a complete <i>GAL4</i> gene (Gal4Full) and the negative control yeast express the <i>GAL4</i> DNA binding domain alone (Gal4DBD). (A) Blue color on SD-Trp with X-α-Gal indicates expression of the <i>MEL1</i> reporter gene. (B and C) Growth on the SD-His and SD-Ade plates indicates expression of the <i>HIS3</i> and <i>ADE2</i> reporter genes, respectively, and are essential for cell viability.