Transcriptional activation analysis of ABS5/T5L1 and ABS7/MYB101 in yeast.

<p>Yeast strain AH109 was transformed with a negative control vector (pBD), a positive control pBD-WRKY33, pBD-ABS5 or pBD-ABS7, respectively. Each of the BD vectors was co-transformed with an empty AD vector, pGADT7. <b>A–C</b>. Growth of yeast transformants on the SD/-Trp-Leu medium (<b>A</b>), the SD/-Trp-Leu-His medium plus 5 mM 3-AT (<b>B</b>) or the SD/-Trp-Leu-His-Ade medium (<b>C</b>). <b>D</b>. Activation of the <i>LacZ</i> gene analyzed via filter lifting X-β-gal assays.