Withanolide A mediated restoration of endogenous glutathione level reverses hypoxia induced neuronal apoptosis in hippocampus.

<p>Administration of withanolide A prior to and during hypoxic exposure decreases number of apoptotic cells in CA3 region of hippocampus while glutathione depletion using buthionine sulfoximine elevates hypoxic neuronal apoptosis. Co-administration of withanolide A alongwith buthionine sulfoximine during hypoxic exposure enhances hypoxia induced apoptotic cells attenuating the nuroprotective effect of withanolide A. Data expressed as percentage change taking normoxic value as 100% and represents Mean ± SEM ‘a’ denotes p≤0.05 vs. when compared to normoxic group. ‘b’ denotes p≤0.05 vs. when compared to normoxia + withanolide A group and ‘c’ denotes p≤0.05 vs. when compared to hypoxia + vehicle group, ‘d’ denotes p≤0.05 vs. when compared to hypobaric hypoxia + withanolide A, ‘e’ denotes p≤0.05 vs. when compared to normoxia + buthionine sulfoximine, and ‘f” denotes p≤0.05 vs. when compared to hypobaric hypoxia + buthionine sulfoximine.