KY-05009 inhibits TGF-β1-mediated modulation of EMT markers.

<p>The effect of KY-05009 on TGF-β1-mediated modulation of EMT markers was evaluated by (A) Western blot analysis and (B) immunofluorescence microscopy. Serum-deprived A549 cells were treated with TGF-β1 or its combination with KY-05009 for 48 h. Actin was used as a loading control. Nuclei were counterstained with Hoechst 33342, and all scale bars represent 20 µm. The expressions of E-cadherin and vimentin were represented by the relative intensity of green fluorescence (n = 30), and reported images are representatives of triplicate experiments. ^##<i>p</i><0.01,* <i>p</i><0.05 (versus ‘the group treated with TGF-β1 only’).