Inhibitory effect of AT₂R on TGF-β1-mediated EMT in HK-2 cells.

<p>HK-2 cells were pretreated with CGP42112A at the indicated dose for 1 h and then treated with TGF-β1 (5 ng/mL) for 24 h. (A) CGP42112A attenuated the morphologic changes induced by TGF-β1 in HK-2 cells (Magnification 100×). (B and C) Pretreatment with CGP42112A abrogated TGF-β1-induced α-SMA expression (B) and restored E-cadherin expression (C). Results are expressed as the ratio of TGF-βRII to GAPDH densities (n = 4–5, *<i>P</i><0.05 vs. control, one-way factorial ANOVA followed by Holm-Sidak post-hoc test). (D and E) Expression of α-SMA (D) and E-cadherin (E) in HK-2 cells treated with CGP42112A at the indicated dose. Results are expressed as the ratio of α-SMA or E-cadherin to GAPDH densities (n = 4). (F and G) Immuofluorescence staining of α-SMA (F) and E-cadherin (G) in HK-2 cells.