TGFβ1 induces EMT through SMAD-3 phosphorylation, which was prevented by AqE, AlE, CA and CI.

<p>Effect of LY364947 and SIS3 on the expression of EMT markers altered by TGFβ1, seen by Quantitative real time PCR (A), Immunofluorescence (B), ELISA (C), Western blot showing TGFβ1 induction of SMAD-3 phosphorylation at various time points (D) effect of AqE, AlE, CA and CI on TGFβ1 induced SMAD-3 phosphorlyation at 30 min (E). Data represents mean ± SD of three independent experiments. p value: ^##p<0.01 is a comparison between control and TGFβ1 treatment. p values: *p<0.05, **p<0.01 are a comparison between TGFβ1 and corresponding treatment.