Protein sources affect energy expenditure and tend to affect spontaneous locomotor activity.

<p>A: RER in mice fed LF diet for 72 h and HF diets for 72 h in open-circuit indirect calorimetry cages. B: Average respiratory exchange ratio (RER) during 48 h on LF diet and HF diets in light and dark phases. C: Spontaneous locomotor activity during 72 h on LF diet and 72 h on HF diets. D: Spontaneous locomotor activity in light and dark phases during 48 h in mice fed LF diet and HF diets. E: Total spontaneous locomotor activity during 48 h in mice fed LF diet and HF diets. F: Energy expenditure (EE) during 72 h on LF diet and 72 h on HF diets. G: Average EE during 48 h in light and dark phases in mice fed LF diet and HF diets. Data (Expt. 2) represent group means (<i>n</i> = 9–10) ± SEM analyzed by ANOVA followed by Tukey's pair-wise comparisons. RER, activity and EE data were analyzed by repeated measurements ANOVA followed by Tukey's post hoc. Means that do not share a letter are significantly different (<i>P</i><0.05).