Investigation of tissue-lineage specificity of trypsinized and re-cultured hMSCs (re-cultured in osteo- and adipogenic induction medium).

<p>Trypsinized hMSCs (group 3) were re-cultured in osteogenic (<b>a, b, e, f</b>) and adipogenic medium (<b>c, d, g, h</b>) for 7 days and lineage commitment was validated by detecting the tissue-lineage specific markers; β-MHC, osteocalcin along with oil red O dye. Immunostaining results proved that trypsinized and re-cultured patterned cells exhibited cardiac MHC expression (<b>a, c</b>) in osteo- and adipogenic medium respectively, whereas these re-cultured patterned cells failed to express osteogenic marker (<b>b</b>) or to produce oil globules (<b>d</b>). Trypsinized and re-cultured unpatterned cells exhibited no traces of cardiac marker expression (<b>e, g</b>) but showed osteocalcin expression (<b>f</b>) and tiny oil globules formation (<b>h</b>). The scale bar is 100 µm.