Validation of tissue-lineage commitment of hMSCs.

<p>Tissue-lineage specific markers; osteocalcin and β-MHC along with oil red O dye were used to check hMSCs commitment grown in osteogenic (<b>a, b, e, f</b>) and adipogenic medium (<b>c, d, g, h</b>) for 14 days. Immunostaining results revealed that patterned cells displayed osteocalcin expression (<b>a</b>) and oil globules formation (<b>c</b>) cultured in osteo- and adipogenic medium respectively, while patterned cells grown in osteo- and adipogenic medium failed to express cardiac marker (<b>b, d</b>). Unpatterned cells followed similar trend by showing osteocalcin expression (<b>e</b>), distinct oil globules formation (<b>g</b>) and no indication of β-MHC expression (<b>f, h</b>). The scale bar is 100 µm.