Differentiation of transduced hMSCs.

<p>Transduced hMSCs were incubated in either adipogenic or osteogenic differentiation medium in the presence of doxycycline. The cells were fixed with 4% paraformaldehyde and stained with Oil Red O for adipogenic cells (C and D) or Alizarin Red for osteogenic cells (E and F) 3 weeks in vitro. (A) Undifferentiated hMSCs expressed hrGFP. (B) A phase contrast photomicrography of A. (C) Transduced hMSCs differentiated into adipogenic cells versus undifferentiated transduced hMSCs (D). (E) transduced hMSCs differentiated into osteogenic cells versus undifferentiated transduced hMSCs (F). The scale bar = 50 µm.