Verification of tissue-lineage commitment of trypsinized and re-cultured hMSCs (re-cultured in normal growth medium).

<p>Trypsinized hMSCs were re-cultured in normal growth medium for 7 days and subjected to immunostaining procedure. Trypsinized cells maintained their myocardial lineage commitment and it was confirmed by β-MHC positive immuno-labeling (<b>a</b>) and no osteocalcin expression (<b>b</b>). Contrary to that, trypsinized unpatterned cells showed down regulation of β-MHC (<b>c</b>) and predominant expression of osteocalcin (<b>d</b>).