Effect of PTB over-expression on JEV replication in Vero cells.

<p>(a) Vero cells were transfected with pcPTB and levels of PTB checked by Western blotting 48 h later. (b) Vero cells transfected with pcPTB or pcDNA were infected 43 h later with JEV at a moi of 0.1 and 1. The culture supernatants were harvested at different time points and JEV titers determined. Mean virus titers and standard deviation from the experiment done in triplicates are shown. JEV titers in PTB over-expressing cells were consistently inhibited as indicated at the bottom of the figures.