PTB colocalization with dsRNA in JEV-infected cells.

<p>Monolayers of Vero cells were infected with JEV at a moi of 10. At the indicated time pi, the cells were fixed, permeabilized, and stained with mouse dsRNA antibody, rabbit NS3 and NS5 antibodies (panel a), goat PTB antibody (panel b), followed by staining with anti-goat Alexa 568, anti-mouse Alexa 488, and anti-rabbit Alex 568 secondary antibodies. Cells were visualized after adding mounting medium with DAPI to stain the nuclei. Localization of dsRNA is shown with green fluorescence whereas PTB, NS3 and NS5 are shown with red fluorescence. Colocalization of dsRNA with PTB results in yellow fluorescence as seen in the merge panels.