Effect of PTB knock-down on JEV replication in cultured cells.

<p>(a) Vero cells were transfected with plasmid encoding shRNA against PTB (sh-PTB) or Luciferase (sh-Luc) and analysed 48 h later by Western blotting for PTB levels. (b) Vero cells transfected with shRNA-encoding plasmids were infected 43 h later with JEV at a moi of 0.1 and 1. The culture supernatants were harvested 24 h pi and JEV titers determined. Mean virus titers and standard deviation from the experiment done in triplicates are shown.