<p>Eight white colonies were screened for each construct. Non-purified PCR products were used in this study. Efficiency indicates % of clones that contained the expected insert.</p><p>PCR screening of colonies from nine Hot Fusion transformation plates.</p
Author: Matt Lewis ### Notes This is the fastest way to screen bacterial colonies. Our PCR machine...
A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym ...
Broad spectrum producing hosts and fundamental research models such as yeast can only be developed ...
The paper describes a simple and effective protocol for screening recombinant clones, which can be u...
<p>(A) A schematic diagram for the creation of a seamless construct. The vector was linearized by Sp...
Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on sel...
After the regeneration of protoplasts and two rounds of selection on antibiotic-containing medium th...
<p>Analysis I: colonies from cm-plates (all grow) amplified with WO375 & B1 (all amplified) and dige...
<p>(<b>A</b>) PCR product amplified from G-tailed cDNA prepared from biopsy sample T019 using primer...
<p>Agarose gel images after colony PCR showing, (A) clone-3 to be positive in pMS-QS-NOHGFP; (B) clo...
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
DNA from the wild-type strain (W), 94 transformants, and the plasmid pLF5HA, which contains the LF5-...
<p><b>Copyright information:</b></p><p>Taken from "A dual-fluorescence reporter system for high-thro...
<p>Predicted fragment sizes (approximate, in bp) and PCR cycle profiles (temperature in °C: time in ...
<p>PCR was performed with the indicated primer sets in Materials and methods. The type of genome-int...
Author: Matt Lewis ### Notes This is the fastest way to screen bacterial colonies. Our PCR machine...
A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym ...
Broad spectrum producing hosts and fundamental research models such as yeast can only be developed ...
The paper describes a simple and effective protocol for screening recombinant clones, which can be u...
<p>(A) A schematic diagram for the creation of a seamless construct. The vector was linearized by Sp...
Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on sel...
After the regeneration of protoplasts and two rounds of selection on antibiotic-containing medium th...
<p>Analysis I: colonies from cm-plates (all grow) amplified with WO375 & B1 (all amplified) and dige...
<p>(<b>A</b>) PCR product amplified from G-tailed cDNA prepared from biopsy sample T019 using primer...
<p>Agarose gel images after colony PCR showing, (A) clone-3 to be positive in pMS-QS-NOHGFP; (B) clo...
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
DNA from the wild-type strain (W), 94 transformants, and the plasmid pLF5HA, which contains the LF5-...
<p><b>Copyright information:</b></p><p>Taken from "A dual-fluorescence reporter system for high-thro...
<p>Predicted fragment sizes (approximate, in bp) and PCR cycle profiles (temperature in °C: time in ...
<p>PCR was performed with the indicated primer sets in Materials and methods. The type of genome-int...
Author: Matt Lewis ### Notes This is the fastest way to screen bacterial colonies. Our PCR machine...
A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym ...
Broad spectrum producing hosts and fundamental research models such as yeast can only be developed ...
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