Diagnostic polymerase chain reaction (PCR) assay of the variants obtained from the combinatorial screening.

<p>PCR was performed with the indicated primer sets in Materials and methods. The type of genome-integrated cellulase construct was determined from the lengths of the PCR products. PCR products of the transformants carrying the CBH1 (A, B), CBH2 (C, D), and EG2 constructs (E, F). The PCR products for the core promoter (A, C, E) and terminator regions (B, D, F) were amplified.