<p><b>Copyright information:</b></p><p>Taken from "A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting"</p><p>Nucleic Acids Research 2005;33(5):e49-e49.</p><p>Published online 14 Mar 2005</p><p>PMCID:PMC1065264.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> All 46 cultures yielded a PCR product consistent with a cloned insert. The majority of these inserts were in the 1.5–3 kb size range selected during initial library construction
Changes of bacterial profiles in microbial communities are strongly associated with human health. Th...
<p>An agarose gel is depicted. The arrow indicates the molecular size after IS10 insertion. T0, DNA ...
aPlasmid copy number (PCN) was determined after induction of the donor intron. Total genomic and pla...
<p>Lanes labeled 1 to 21 give examples of individual clones that were randomly picked out from the c...
<p>Genomic DNA or BAC DNA isolation and purification was followed by size fractionation and ligation...
<p>Hyperladder in lanes 1 and 25 allows estimation of insert length. PCR controls in lanes 22, 23 an...
<p>Eight white colonies were screened for each construct. Non-purified PCR products were used in thi...
<p>A: scatter-plot of cloned insert size and total well GFP fluorescence in transfected HEK293T cell...
The red rectangles on top of gels A and B represent the cropped areas used to build Fig 1N and 1P (B...
The method to internally label PCR products with multiple colored fluorescent dyes was developed and...
<p>Lanes 1, 2, 3 and 4 in (A), (B) and (C) show the products of PCR amplification of cDNA templates ...
<p><b>Copyright information:</b></p><p>Taken from "Combinatorial expression vector engineering for t...
One of the fundamental techniques of molecular biology is the isolation of a rare clone from a compl...
<p><b>A.</b> PCR using genomic DNA as template for four different PCR (PCR1 = 645bp, PCR3 = 517 bp, ...
DNA from the wild-type strain (W), 94 transformants, and the plasmid pLF5HA, which contains the LF5-...
Changes of bacterial profiles in microbial communities are strongly associated with human health. Th...
<p>An agarose gel is depicted. The arrow indicates the molecular size after IS10 insertion. T0, DNA ...
aPlasmid copy number (PCN) was determined after induction of the donor intron. Total genomic and pla...
<p>Lanes labeled 1 to 21 give examples of individual clones that were randomly picked out from the c...
<p>Genomic DNA or BAC DNA isolation and purification was followed by size fractionation and ligation...
<p>Hyperladder in lanes 1 and 25 allows estimation of insert length. PCR controls in lanes 22, 23 an...
<p>Eight white colonies were screened for each construct. Non-purified PCR products were used in thi...
<p>A: scatter-plot of cloned insert size and total well GFP fluorescence in transfected HEK293T cell...
The red rectangles on top of gels A and B represent the cropped areas used to build Fig 1N and 1P (B...
The method to internally label PCR products with multiple colored fluorescent dyes was developed and...
<p>Lanes 1, 2, 3 and 4 in (A), (B) and (C) show the products of PCR amplification of cDNA templates ...
<p><b>Copyright information:</b></p><p>Taken from "Combinatorial expression vector engineering for t...
One of the fundamental techniques of molecular biology is the isolation of a rare clone from a compl...
<p><b>A.</b> PCR using genomic DNA as template for four different PCR (PCR1 = 645bp, PCR3 = 517 bp, ...
DNA from the wild-type strain (W), 94 transformants, and the plasmid pLF5HA, which contains the LF5-...
Changes of bacterial profiles in microbial communities are strongly associated with human health. Th...
<p>An agarose gel is depicted. The arrow indicates the molecular size after IS10 insertion. T0, DNA ...
aPlasmid copy number (PCN) was determined after induction of the donor intron. Total genomic and pla...