Phenotype changes in SH-SY5Y cells in response to rotenone treatment.

<p>The dose (A) and time (B) responses of NO generated in SH-SY5Y cells that were treated with rotenone were analyzed. 18h treatment (A) and 500nM rotenone (B) were used for the gradient experiments. The NO contents are represented as the ratio of the intensity of DAF-FM fluorescence in the rotenone-treated group compared with the vehicle-treated group (average ratio ± SEM, n = 3, *P<0.05). Apoptosis assessment by flow cytometry for SH-SY5Y cells treated with or without 500nM rotenone for 16h (C). Dot plot showed annexin V-FITC in x-axis and PI in y-axis. Cells in the fourth quadrant undergoing early stage apoptosis are annexin V-positive/PI negative. And cells at late stage apoptosis or necrotic cells are both annexin V-FITC and PI positive. The left represents the untreated cells as the control. The apoptotic rates are shown as the average ratio ± SEM (n = 3).