Super-resolution imaging of CHMP2A at HIV-1 assembly sites.

<p>HeLa cells were transfected with HIV:HIV^mCherry and endogenous CHMP2A was detected by immunostaining 14–15 h post transfection. HeLa cells were imaged using TIRFM. (<b>A</b>) <i>Left panel</i>. A merged time-projected TIRF image of HIV^mCherry (magenta) and CHMP2A (green) prior to STORM analysis. <i>Middle panel</i>. A zoomed-in image of the selected CHMP2A cluster highlighted in grey in the left panel colocalizing with an HIV assembly site. <i>Right panel</i>. The corresponding drift-corrected STORM image of the CHMP2A cluster. Scale bars: 500 nm. (<b>B</b>) The size distribution of all CHMP2A structures colocalizing with HIV^mCherry with an average cluster size (FWHM) of 56 ± 12 nm. (<b>C</b>) Size distribution of all non-colocalizing CHMP2A clusters in cells co-expressing HIV^mCherry with an average cluster size (FWHM) of 59 ± 20 nm.