CD4 (LAMP and STOP) intracellular trafficking guides HIV intracellular localization to lysosomal compartments.

<p>CD4-transduced HEK cells incubated with GFP HIV for 2–4 hours. Confocal images showing representative staining of CD4-transduced HEK cells, where(-) is HEK not transduced with CD4, WT is CD4-WT, STOP is CD4-STOP, DEC is CD4-DEC, LAMP is CD4-LAMP incubated (A) LAMP-1 and (C) lysotracker with GFP HIV for a single confocal z stack with overlay of paired images, scale bar = 20μm and inset (3X). Graphs depict % colocalization (Manders coefficient) for 50–100 cells of GFP HIV (HIV) with (B) LAMP-1 mean ± SD WT 21.59% ± 13.62% as compared to STOP 26.21% ±17.71% as compared to DEC 69.25% ± 20.70% as compared to LAMP 70.01% ± 18.93%, unpaired Student’s t test *p<0.01, comparing WT to STOP, WT to DEC, and WT to LAMP. (D) % colocalization of HIV with lysotracker mean ± SD in WT 23.35% ± 16.64%, in STOP 17.93% ±19.36%, in DEC 62.45% ± 20.84%, in LAMP 68.21% ± 19.67%, unpaired Student’s t test *p<0.01 comparing WT to STOP, WT to DEC, and WT to LAMP.