Captured HIV-1 particles co-localize with CD169 on DCs.

<p>Mature DCs were either (A) mock infected or exposed to Gag-mCherry VLP for (B) <10 minutes, (C) 120 minutes, or (D) 60 minutes with an additional 60 minute co-culture with autologous CD4⁺ T cells. Representative deconvolved maximum intensity images are shown. Top row = Gag-mCherry (red), second row = CD169 (green), third row = composite images (T cells in blue), bottom row = bright-field. Co-localization is reported as the average Mander's coefficient ± SD. M1 = ratio of Gag-mCherry associated with CD169, M2 = ratio of CD169 that has associated Gag-mCherry.