HIV-2 uncoating images.

<p>(A) Images of empty vector-transfected HeLa cells infected with fluorescently labeled HIV-2. The infected cells were fixed at 1 hr after infection. HIV-2 capsids (CA) in cells were probed with Cy5 using anti-HIV-2 p26 antibodies. Fluorescent images of GFP-Vpx (Vpx, green), S15-Tomato (S15, red), HIV-2 p26 (p26, blue), a merged image of these three colors (Merge), a binary image of GFP-Vpx (Bi, green), and a merged image of Bi, S15, and p26 (Bi+S15+p26) are shown. In this image, we detected one particle with red, green, and blue three-fluorescent co-localization (thin arrow); 4 particles with green and blue two-fluorescent co-localization (one of them is indicated by a thick arrow); and nine particles with green fluorescent only (one of them is indicated by an arrow head). A bar shows 10 μm. (B) Images of HeLa cells stably expressing cynomolgus monkey TRIM5α following infection with fluorescently labeled HIV-2. Layout of the images is as described in the legend for panel A. In this image, we detected no particles with red, green, and blue co-localized fluorescence; two particles with green and blue co-localized fluorescence; and 13 particles with green fluorescence only.