Expression of recombinant proteins in <i>E</i>. <i>coli</i>.

<p>A) Size exclusion chromatogram showing monomeric nature of wt and mutated proteins. B) The purity and quality of the mutants was assessed by electrophoresis: 2 μg of wt and mutant proteins were run on 12% SDS-PAGE gel under reducing conditions and stained with Coomassie. C) Far CD spectra of the proteins studied herein, showing nearly identical secondary structures for all the mutants. For color coding and mutation see Table I.