Summary of the recombinant genes and the proteins purified from <i>E</i>.<i>coli</i>.

<p>A: Schematic representation of the combinations of E2 fragments. Red- main early neutralizing epitope; yellow- surface exposed domains of A; red/white—one peptide of L; orange- domain B with ß-ribbon connector. B: Overview of the recombinant proteins purified by Ni²⁺ affinity chromatography from <i>E</i>.<i>coli</i>. Proteins were separated on a 15% SDS-PAGE and stained with Coomassie. The prominent faster migrating bands of protein LB⁺ were identified by mass spectrometry as degradation products of LB⁺ (see <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003684#pntd.0003684.s003" target="_blank">S3 Fig</a>). Lanes 1–5 from the left were loaded with the indicated amounts of BSA and used to estimate the protein amount of the recombinant proteins.