<p>bmGSTT mutants were prepared by site-directed mutagenesis. Purified recombinant proteins were applied to SDS-PAGE followed by Coomassie Brilliant Blue R250 staining; M, protein molecular size markers; lane 1, WT; lane 2, H40A; lane 3, E66A; lane 4, S67A; lane 5, R107A; and lane 6, V54A.</p
<p><b>Copyright information:</b></p><p>Taken from "Directed evolution of single-chain Fv for cytopla...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>A: Schematic representation of the combinations of E2 fragments. Red- main early neutralizing epi...
Purified wild-type (WT) recombinant Nus-BB0562, single (S34A; S34T; S58A; S58T) and double mutant (S...
<p>A) Size exclusion chromatogram showing monomeric nature of wt and mutated proteins. B) The purity...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>(A) Coomassie brilliant blue (CBB) staining of recombinant AmJHAMT. Samples were: lane 1 (negativ...
<p>GST and GST-ST1, -ST2 and -ST3 fusions proteins were expressed in <i>E.coli</i>, extracted, and r...
<p>(A), insoluble and soluble fractions of BPSM (wt control; lanes 1 and 4), BPRM1 (deletion mutant;...
<p>(a) SDS-PAGE analysis of the purified recombinant VpPR-10.1 protein. Lane 1, protein marker; lane...
<p>The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...
<p>Lane 1 showed the BL-PET30 as negative control; Lane 2~4 and lane 6~10 showed different recombina...
<p>Expression and purification analysis of recombinant proteins Lsa46 <b>(A)</b> and Lsa77 <b>(E)</b...
<p>Expression of BVDV-E2 in <i>E</i>. <i>coli</i> BL21 (DE3). When DE3 bacteria reached the logarith...
<p>The N-terminal (first), middle (second), and C-terminal (third) dsRBDs were denoted as dsRBD A, d...
<p><b>Copyright information:</b></p><p>Taken from "Directed evolution of single-chain Fv for cytopla...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>A: Schematic representation of the combinations of E2 fragments. Red- main early neutralizing epi...
Purified wild-type (WT) recombinant Nus-BB0562, single (S34A; S34T; S58A; S58T) and double mutant (S...
<p>A) Size exclusion chromatogram showing monomeric nature of wt and mutated proteins. B) The purity...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>(A) Coomassie brilliant blue (CBB) staining of recombinant AmJHAMT. Samples were: lane 1 (negativ...
<p>GST and GST-ST1, -ST2 and -ST3 fusions proteins were expressed in <i>E.coli</i>, extracted, and r...
<p>(A), insoluble and soluble fractions of BPSM (wt control; lanes 1 and 4), BPRM1 (deletion mutant;...
<p>(a) SDS-PAGE analysis of the purified recombinant VpPR-10.1 protein. Lane 1, protein marker; lane...
<p>The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...
<p>Lane 1 showed the BL-PET30 as negative control; Lane 2~4 and lane 6~10 showed different recombina...
<p>Expression and purification analysis of recombinant proteins Lsa46 <b>(A)</b> and Lsa77 <b>(E)</b...
<p>Expression of BVDV-E2 in <i>E</i>. <i>coli</i> BL21 (DE3). When DE3 bacteria reached the logarith...
<p>The N-terminal (first), middle (second), and C-terminal (third) dsRBDs were denoted as dsRBD A, d...
<p><b>Copyright information:</b></p><p>Taken from "Directed evolution of single-chain Fv for cytopla...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>A: Schematic representation of the combinations of E2 fragments. Red- main early neutralizing epi...
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