Figure 6

<p>(<b>A</b>) Crosslinking of mutant p24:V3 chimera. Proteins were separated in non reducing SDS-PAGE conditions on a 10% polyacrilamide gel. The p24:V3 CC and CCAA mutants migrate almost exclusively as hexamers, while the p24:V3 WT chimera migrates as monomer. (<b>B</b>) Size exclusion chromatographic profile. Profile of p24:V3 WT (gray) and p24:V3 CCAA mutant (black) on Superdex-200 PC column is shown. Elution volumes of protein standards such as ferritin (440 kDa,dashed and dotted line) and aldolase (158 kDa, dotted line) are also indicated.