PbPL-knockout parasites take longer to appear in the blood after sporozoite infection.

<p>C57BL/6 mice were injected intravenously with 1,000 wild-type (WT), PbPL-knockout (KO2) or complemented PbPL-KO (CMP2) sporozoites and subsequent blood stage parasitemia was measured by FACS analysis. A) Blood stage parasitemia at day 4. B) Development of parasitemia between day 3 and 6 post-infection. Shown are means +/− SD of 6–7 infected mice per group. For statistical analysis of parasitemia at day 4, a one-way analysis of variance (ANOVA) followed by a Holm-Sidak multiple comparison test was performed (** p < 0.01, n.s. = not significant). See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004760#ppat.1004760.s003" target="_blank">S3 Fig.</a>