Parasite burden and parasitemia following vaccination and infection.

<p>Mice were vaccinated s.c. three times at three week intervals with lipid core peptides (LCP), or pooled peptides (P1, P2, P3, P4; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040928#pone-0040928-t001" target="_blank"><i>Table 1</i></a>) and CpG-Alum, or i.m. injection with DNA vaccine encoding <i>Py</i>CSP. Seven days after the final vaccination mice were challenged by i.v. injection with live <i>P. yoelii</i> sporozoites (SPZ inf) or parasitized red blood cells (Blood inf). (A) Liver parasite burden in sporozoite infected mice, assessed 42 h post infection by RT-PCR of parasite 18S rRNA (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040928#s2" target="_blank"><i>Materials and Methods</i></a>) (n = 5 mice, mean and SEM). (B) Kinetics of parasitemia and (C) area under curve of parasitemia (AUC); results of pooled data from three repeat experiment are shown (n = 21 mice, mean and SEM). Statistical comparisons are made to a control group that received no vaccination (No Vacc Ctrl) using one-way ANOVA with Bonferroni's post-test.