Effect of missense mutations in STING found in cancer cells on activation of the IFNβ promoter.

<p>(A) Luciferase reporter assay for HEK293T cells cotransfected with pcDNA3, wild-type STING, or STING missense mutants, and an IFNββ promoter reporter construct, pLUC- IFNβ. 5 ng (5) or 25 ng (25) of STING expression construct was transfected as indicated. The normalized relative luciferase activity activity is shown (mean +/- standard deviation) (B) Luciferase reporter assay for HEK293T cells cotransfected with pcDNA3, wild-type STING, or R284M mutant, and the pLUC-PRD(III-I)₃ or pLUC-PRD(II)₂ reporter construct as indicated.