DENV NS2B3 inhibits induction of IFNβ and p55-C1B promoters by human STING.

<p>(A) 293T cells were co-transfected with a vector encoding for an IFNβ promoter driving the luciferase gene, a plasmid encoding for human STING, and plasmids for different versions of DENV protease (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002934#ppat-1002934-g001" target="_blank">Fig. 1D</a>). Luciferase activity was measured 24 hours after transfection. (B) Same as described in (A) but using a plasmid encoding for the mouse version of STING. (C) 293T cells were co-transfected with a vector encoding for p55-C1B promoter driving the luciferase gene, a plasmid encoding for the human STING and plasmids for three different version of the DENV protease (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002934#ppat-1002934-g001" target="_blank">Fig. 1D</a>). (D) Same as described in (C) but using a plasmid encoding for the mouse version of STING. (E) Scheme of the luciferase expressing vectors used, harboring the promoters for IFNβ and p55-CIB (F) Scheme of experimental design. MDDCs were treated with MOCK, DENV and UV inactivated DENV. After 12 hours, the three sets of cells were stimulated with MOCK or purified <i>E. coli</i> DNA and total RNA was purified at 2 and 7 hours post treatment. Relative expressions of type I IFN and ISGs, normalized against rsp11 and α-tubulin, were measured by qRT-PCR at specific time points. (G) INFβ mRNA. (H) IFNα mRNA. (I) ISG15 mRNA. Data shown represents one of two independent experiments. Error bars are the standard deviation of two individual replicates. <b>*</b>, p<0.05.