Cytokine mRNA expression by MLN and spleen cells after <i>in vitro</i> stimulation with TsCE and rTsCRT.

<p>cDNA was prepared from mRNA obtained from MLN (A, C, D) or spleen (B, D, E). mRNA levels of IL-4 (A, B) IL-5 (C, D) and IL-10 (E, F) were determined by quantitative RT-PCR. Data are expressed as the mean of the ratio of each cytokine relative to β-actin (housekeeping gene). White bars represent data from cells incubated in RPMI alone, grey bars from cells incubated with rTsCRT and hatched bars with TsCE. Bars represent mean ± SEM of relative expression from triplicate cultures of cells obtained from 6 animals. Kruskal-Wallis: * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 rTsCRT-induced expression as compared to the RPMI control. ^# P<0.05, ^## P<0.01 rTsCRT-induced expression as compared to TsCE. ND, not detected.