Antiinflammatory cytokine mRNA expression in colon and MLN determined by qRT-PCR.

<p>mRNA levels of IL-4 in colon (A) and MLN (B); IL-10 in colon (C) and MLN (D) and IL-13 in colon (E) and MLN (F). Data are expressed as the mean of the ratio of each cytokine relative to β-actin (housekeeping gene). Open bars represent data from control mice; grey bars from mice orally immunized with rTsCRT; black bars from mice treated with TNBS; hatched bars from mice orally immunized with rTsCRT and treated with TNBS and dotted bars from mice treated with ethanol. Kruskal-Wallis test was used to analyze relative expression in colon and one-way ANOVA for MLN. Results are expressed as means + SEM of pooled data from two separate experiments; n = 12,13 mice per group. *P<0.05; **P<0.01; ***P = 0.001.