Cytokine analysis by RT-PCR.

<p>Relative transcription of Th1, Th2, pro-inflammatory, anti-inflammatory, Th17 and regulatory cytokines in PBMCs isolated from animals bled at various time points after immunization and stimulated with/without Ag-mix (15 μg/ml) or PPDj (10 µg/ml) for 3 days was measured by RT-PCR. The relative transcription was calculated using the value of unstimulated cells as the calibrator with the housekeeping gene GAPDH as an internal control. Data are presented as the relative mRNA expression (mean fold change) and the mean value of each group. The error bars indicate standard deviation from the mean. (PBS Vs 316F *indicates p<0.05 ** indicates p<0.01, PBS Vs Sal-Ag <b>‡</b> indicates p<0.05 <b>‡‡</b> indicates p<0.01).