Relative expression (fold-change) of immune genes in mitogen-stimulated splenic lymphocytes.

Splenic lymphocytes were stimulated with the mitogens (5 μg/mL of Con A or 10 μg/mL of LPS). After 48 h stimulation, total RNA was extracted from the stimulated cells. The cytokine expression was analyzed using the QuantStudio™ 7 FlexReal-Time PCR System. (A) IL-1β, (B) IL-2, (C) IL-4, (D) IL-6, (E) IL-10, (F) TNF-α, (G) IFN-γ, (H) TLR-4, and (I) COX-2 genes. Significant differences were observed at P .05 compared with the saline (*) and ginseng (#) group.