SDS sensitivity of <i>E</i>. <i>coli tat</i> mutant strains expressing the <i>C</i>. <i>glutamicum</i> TatB protein from plasmid pHSG-TatB^CG.

<p>MC4100 (wild-type) containing the empty vector pHSG575 (black squares), BØD (Δ<i>tatB</i>) containing pHSG575 (green circles), BØD containing pHSG-TatB^EC (pink triangles), BØD containing pHSG-TatB^CG (purple asterisks), JARV15 (Δ<i>tatA/E</i>) containing pHSG575 (blue diamonds), and JARV15 containing pHSG-TatB^CG (red open pentagons) were analyzed for SDS sensitivity using the assay described by Ize et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123413#pone.0123413.ref040" target="_blank">40</a>]. The cells were grown for 3 h in LB medium containing 100 μM IPTG in the presence of various SDS concentrations as outlined in the Material and Methods section. 100% survival is defined as the optical density of each strain after 3 h growth in LB medium without SDS. The experiments were performed in triplicate using biologically independent replicates and the respective standard deviations are indicated.